基于美洲蜚蠊下颚须的整合受体生物传感器测定抗坏血酸

报道了以美洲蜚蠊的下颚须作为分子识别和转换元件研制的一种新型生物组织传感器,发现该传感器对抗坏血酸(AA) 有选择性的响应,响应脉冲的发放频率与AA 的浓度对数成正比,在5 ×10 - 5至5 ×10 - 2 mol/ L 范围内呈线性关系,检测下限低于10 - 6 mol/ L AA. 该传感器响应快,并具有较好的重现性.
关键词　生物传感器,化学受体,美洲蜚蠊,下颚须
Abstract 　An intact2chemoreceptor2based biosensor based on the gustatory organ , the maxillary palp of Periplaneta americana , is reported , in which the maxillary palp serves as not only the molecular recognition elements but also the biological transducers. The binding events between ligand and chemoreceptor induce action potentials (APs) whose frequency correlates directly to the concentration of stimulants. The APs were recorded with a three2electrode system and collected by sound card method developed by our group. This is the first time that the biosensor is reported to yield quantitative linear dose2response data over the concentration range from 5 ×10 - 5 to 5 ×10 - 2 mol/ L. The new biosensor possesses some advantages over the biosensor based on isolated receptor : shorter response time ( < 1 s) , higher degrees of sensitivity (detect2limitation below 10 - 6 mol/ L) , broader dynamic response range (over four orders of magnitude) and more preferable reproducibility.
Keywords 　chemoreceptor , biosensor , Periplaneta americana , maxillary palp